生物系研究部第2生物活性研究部

研究業績

Thiazoplanomicin, a new thiazolyl peptide antibiotic from the leaf-litter actinomycete Actinoplanes sp. MM794L-181F6.
Y. Takehana, H. Muramatsu, M. Hatano, Y. Ishizaki, M. Umekita, Y.Shibuya, C. Hayashi, T. Kimura, T. Takeuchi, K. Shimuta, R. Sawa, and M. Igarashi
J. Antibiot., 2024. doi: 10.1038/s41429-024-00783-7.

Pyricularia oryzae enhances Streptomyces griseus growth via non-volatile alkaline metabolites.
R. Sugita, T. Arazoe, T. Motoyama, H. Osada, T. Kamakura, K. Kuramochi, and Y. Furuyama
Environmental Microbiology Reports, 2024, 16:e70012. https://doi.org/10.1111/1758-2229.70012

Identification of a family of species-selective complex I inhibitors as potential anthelmintics
T. Davie, X. Serrat, L. Imhof, J. Snider, I. Stagljar, J. Keiser, H. Hirano, N. Watanabe, H. Osada, and A.G. Fraser
Nat Commun., 2024, 15, 3367. https://doi.org/10.1038/s41467-024-47331-3

Reclassification of a reveromycin-producer and the proposals of Actinacidiphila reveromycinica sp. nov. and Actinacidiphila acidipaludis comb. nov.
H. Komaki, H. Takagi, S. Takahashi, and H. Osada
Biosci Biotechnol Biochem, 2024, 88, 689-695, https://doi.org/10.1093/bbb/zbae032

Direct visualization of ribosomes in the cell-free system revealed the functional evolution of aminoglycoside.
J. Tomono, K. Asano, T. Chiashi, M. Suzuki, M. Igarashi, Y. Takahashi, Y. Tanaka, and T. Yokoyama
J. Biochem., 2024, 175, 587-598. doi: 10.1093/jb/mvae002.

Antiproliferative activities through accelerating autophagic flux by basidalin and its analogs in human cancer cells.
T. Matagawa, Y. Sasazawa, K. Agui, M. Fujimaki, S. Kawano, A. Ogura, KI. Takao, M. Igarashi, and S. Simizu
Bioorg Med Chem Lett., 2024, 104, 129713. doi: 10.1016/j.bmcl.2024.129713.

Synthesis and biochemical characterization of naphthoquinone derivatives targeting bacterial histidine kinases.
T. Ishikawa, Y. Eguchi, M. Igarashi, T. Okajima, K. Mita, Y. Yamasaki, K. Sumikura, T. Okumura, Y. Tabuchi, C. Hayashi, M. Pasqua, M. Coluccia, G. Prosseda, B. Colonna, C. Kohayakawa, A. Tani, JI. Haruta, and R. Utsumi
J. Antibiot., 2024, 77, 522–532, https://doi.org/10.1038/s41429-024-00726-2

Analysis of the valgamicin biosynthetic pathway reveals a general mechanism for cyclopropanol formation across diverse natural product scaffolds.
R. F. Little, F. Trottmann, H. Hashizume, M. Preissler, S. Unger, R. Sawa, H. Kries, S. Pidot, M. Igarashi, and C. Hertweck
ACS Chem. 2024, Biol.https://doi.org/10.1021/acschembio.3c00648

Precursor-directed biosynthesis and biological activity of tripropeptin Cpip, a new tripropeptin C analog containing pipecolic acid.
H. Hashizume, R. Sawa, Y. Kubota, S. Adachi, S. Harada, and M. Igarashi
J. Antibiot., 2024, https://doi.org/10.1038/s41429-024-00703-9

Catenulopyrizomicins, new anti-Hepatitis B virus compounds, from the rare actinomycete Catenuloplanes sp. MM782L-181F7.
M. Yamasaki, R. Sawa, H. Muramatsu, Y. Yamamoto, M. Umekita, Y. Kubota, Y. Kanegae, and M. Igarashi
J. Antibiot., 2024, 77, 85-92. doi: 10.1038/s41429-023-00681-4.

Two new adenopeptins B and C inhibit sphere formation of pancreatic cancer cells.
D. Tatsuda, M. Amemiya, C. Nosaka, R. Sawa, H. Muramatsu, M. Igarashi, J. Yoshida, T. Ohishi, and M. Kawada
https://doi.org/10.1038/s41429-023-00679-y

Antiplasmodial activity evaluation of a bestatin-related aminopeptidase inhibitor, phebestin.
N. R. Ariefta, B. Pagmadulam, M. Hatano, N. Ikeda, K. Isshiki, K. Matoba, M. Igarashi, C. Nihei, and Y. Nishikawa
Antimicrob Agents Chemother., 2023, 67: e0160622. doi: 10.1128/aac.01606-22

Cycloimidamicins, Novel natural lead compounds for translation inhibition in Pseudomonas aeruginosa.
Y. Ishizaki, M. Umekita, R. Arisaka, M. Hatano, T. Kimura, Y. Kubota, Y. Shibuya, C. Hayashi, R. Sawa, and M. Igarashi
J. Antibiot., 2023, 76, 691-698, https://doi.org/10.1038/s41429-023-00656-5

Synthesis of novobiocin derivatives and evaluation of their antigonococcal activity and pharmacokinetics
K. Sasaki, H. Takada, C. Hayashi, K. Ohya, Y. Yamaguchi, Y. Takahashi, M. Igarashi, and M. Shibasaki
Bioorg. Med. Chem. 2023. https://doi.org/10.1016/j.bmc.2023.117381

Aprosamine derivatives active against multidrug-resistant gram-negative bacteria.
Y. Otsuka, E. Umemura, Y. Takamiya, T. Ishibashi, C. Hayashi, K. Yamada, M. Igarashi, M. Shibasaki, and Y. Takahashi
ACS Infectious Diseases, 2023, 9, 886-898

Sparsomycin exhibits potent antiplasmodial activity In vitro and In vivo.
N. R. Ariefta, B. Pagmadulam, C. Nihei, and Y. Nishikawa
Pharmaceutics, 2022, 544, https://doi.org/10.3390/pharmaceutics14030544

Synthesis and antiviral activity of formycin derivatives with anti-influenza virus activity.
H. Takada, N. Takizawa, S. Shibasaki, H. Asaba, M. Igarashi, M. Shibasaki and Y. Takahashi
Bioorganic & Medicinal Chemistry, 2022, 57, https://doi.org/10.1016/j.bmc.2022.116613

Rediscovery of 4-trehalosamine as a biologically stable, mass-producible, and chemically modifiable trehalose analog.
S. Wada, H. Arimura, M. Nagayoshi, R. Sawa, Y. Kubota, K. Matoba, C. Hayashi, Y. Shibuya, M. Hatano, Y. Takehana, S. Ohba, Y. Kobayashi, T. Watanabe, M. Shibasaki, and M. Igarashi
Advanced Biology 2022, 6, 2101309, https://doi.org/10.1002/adbi. 202101309

Anti-influenza virus activity of methylthio-formycin distinct from that of T-705.
N. Takizawa, H. Takada, M. Umekita, M. Igarashi and Y. Takahashi
Front Microbiol. 2022, 13, 802671. doi: 10.3389/fmicb. 2022.802671

Wychimicins, a new class of spirotetronate polyketides from Actinocrispum wychmicini MI503-A4.
T. Kimura, M. Umekita, M. Hatano, C. Hayashi, R. Sawa and M. Igarashi
J. Antibiot. 2022, 75, 535–541. doi.org/10.1038/s41429-022-00560-4

Isolation of new derivatives of the 20-membered macrodiolide bispolide from Kitasatospora sp. MG372-hF19.
Y. Kohda, S. Sakamoto, M. Umekita, T. Kimura, Y. Kubota, R. Arisaka, Y. Shibuya, H. Muramatsu, R. Sawa, S. Dan, M. Kawada, and M. Igarashi.
J. Antibiot., 2022, 75, 77-85. doi.org/10.1038/s41429-021-00492-5

Polyether ionophore kijimicin inhibits growth of Toxoplasma gondii and controls acute toxoplasmosis in mice
A. Leesombun, CI. Nihei, D. Kondoh, Y. Nishikawa
Parasitol. Res., 2022, 121, 413-422. doi: 10.1007/s00436-021-07363-w.

Total synthesis of Hytramycin V, an antibiotic cyclopeptide.
T. Inaba, Y. Ishizaki, M. Igarashi, M. Yoshida, H. Kigoshi
Bulletin of the Chemical Society of Japan. 2021, 94, 1922-1930.

Micromonosporamide a with glutamine-dependent cytotoxicity from micromonospora sp. MM609M-173N6: isolation, stereochemical determination, and synthesis.
T. Takeuchi, M. Hatano, H. Muramatsu, Y. Kubota, R. Sawa and M. Igarashi
Org. Lett., 2021, 23, 7981-7985.

Metacytofilin has potent anti-malarial activity.
A. Leesombun, M. Iijima, B. Pagmadulam, B. Orkhon, H. Doi, K. Issiki, R. Sawa, CI. Nihei and Y. Nishikawa.
Parasitol Int., 2021, 81, doi: 10.1016/j.parint.2020.102267.

Cargo selection in the early secretory pathway of African trypanosomes.
CI. Nihei and M. Nakanishi.
Parasitol Int., 2021, 84, doi: 10.1016/j.parint.2021.102379

Proteolytic processing, maturation, and unique synteny of the Streptomyces hemagglutinin SHA.
Y. Fujita-Yamaguchi, H. Muramatsu, A.Tapia, K. Bagramyan, M. Desai, Y. Takehana, M. Igarashi, Y. Yamaguchi, and M. Kalkum
Microbiol Spectr. 2021, 9, e0076621. doi: 10.1128/Spectrum.00766-21.

The potent protein phosphatase 2A inhibitors aminocytostatins: new derivatives of cytostatin.
S. Tohyama, M. Kawada, H. Muramatsu, M. Hatano, H. Inoue, K. Matoba, R. Sawa, and M. Igarashi
J Antibiot. 2021, 74, 743-751, doi: 10.1038/s41429-021-00455-w.

Saccharobipyrimicin, a new antibiotic from the leaf-litter actinomycete Saccharothrix sp. MM696L-181F4.
Y. Takehana, H. Muramatsu, M. Umekita, C. Hayashi, T. Kimura, R. Sawa, and M. Igarashi
J. Antibiot., 2021, 74, 470-473, doi: 10.1038/s41429-021-00418-1.

Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules.
T. Ishida, H. Yoshimura, M. Takekawa, T. Higaki, T. Ideue, M. Hatano, M. Igarashi, T. Tani, R. Sawa, and H. Ishikawa
Sci Rep. 2021, 11, 6077, doi. org/10.1038/s41598-021-85501-1.

Sealtomicins, new enediyneantibiotics from the deep-sea actinomycete Nomuraea sp. MM565M-173N2
M. Igarashi, R. Sawa, M. Umekita, M. Hatano, R. Arisaka, C. Hayashi, Y. Ishizaki, M. Suzuki and C. Kato
J. Antibiot., 2021, 74, 291-299.

Genome sequence of Lysobacter sp. Strain BMK333-48F3, the producer strain of potent lipopeptide antibiotics of the tripropeptin family.
P. Arlt, H. Hashizume, M. Igarashi and H. Gross
Microb Res Announc. 2021, 10, e00969-21.

New chloptosins B and C from an Embleya strain exhibit synergistic activity against methicillin-resistant Staphylococcus aureus when combined with co-producing compound L-156,602
H. Hashizume, S. Harada, R. Sawa, K. Iijima, Y. Kubota, Y. Shibuya, R. Nagasaka, M. Hatano and M. Igarashi
J. Antibiot., 2021, 74, 80-85.

Antiparasitic antibiotics from Japan
K. Shiomi
Parasitol. Int., 2021, 82, 102298.

Metacytofilin is a potent therapeutic drug candidate for toxoplasmosis
A. Leesombun, M. Iijima, K. Umeda, D. Kondoh, B. Pagmadulam, A. M Abdou, Y. Suzuki, S. Ohba, K. Isshiki, T. Kimura, Y. Kubota, R. Sawa, CI. Nihei, Y. Nishikawa.
The Journal of Infectious Diseases, 2020, 221, 766-774.

An immuno-dot blot assay for screening histidine kinase inhibitors. 1;600:113765.
Kinoshita-Kikuta E, Maruta S, Eguchi Y, Igarashi M, Okajima T, Utsumi R, Kinoshita E, Koike T.
Anal Biochem. 2020 Jul 1;600:113765.

Isolation and characterization of antiprotozoal compound-producing Streptomyces species from Mongolian soils.
B. Pagmadulam, D. Tserendulam, T. Rentsenkhand, M. Igarashi, R. Sawa, C. Nihei and Y. Nishikawa
Parasitology International, 2020, 74. 101961.

A new indole glycoside from Kitasatospora sp. MG372-hF19 carrying a 6-deoxy-α-L-talopyranose moiety.
Y. Kohda, S. Sakamoto, Y. Otsuka, R. Sawa, Y. Kubota, M. Igarashi, H. Muramatsu, M. Iijima and M. Kawada
J. Antibiot., 2020, 73, 167-170. doi.org/10.1038/s41429-019-0258-9.

Semisynthesis of an anticancer DPAGT1 inhibitor from a muraymycin biosynthetic intermediate.
K. Mitachi, S. M. Kurosu, S. Eslamimehr, M. R. Lemieux, Y. Ishizaki, W. M. Clemons,Jr. and M. Kurosu.
Org. Lett., 2019, 21, 876-879.

天然物からの次世代新規抗生物質の探索
五十嵐雅之、高橋良昭
日本化学療法学会雑誌　2019, 67(3), 308-323

Flupyranochromene, a novel inhibitor of influenza virus cap-dependent endonuclease, from Penicillium sp. f28743
M. Yamasaki, M. Igarash, R. Sawa, C. Nosaka, M. Umekita, M. Hatano, T. Kimura, K. Iijima, N. Takizawa, T. Kato, K. Mizumoto, and A. Nomoto
J. Antibiot. 2019, 72, 125-133

Leucinostatin Y: A peptaibiotic produced by the entomoparasitic fungus Purpureocillium lilacinum 40-H-28.
I. Momose, T. Onodera, H. Doi, H. Adachi, M. Iijima, Y. Yamazaki, R. Sawa, Y. Kubota, M. Igarashi and M. Kawada
J. Nat. Prod. 2019, 82, 1120-1127

Synthesis and biological activity of analogs of CPZEN-45, a novel antituberculosis drug.
Y. Ishizaki, Y. Takahashi, T. Kimura, M. Inoue, C. Hayashi and M. Igarashi.
J Antibiot. 2019 Dec;72(12):970-980.

Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes
E. K. Kikuta, H. Kusamoto, S. Ono, K. Akayama, Y. Eguchi, M. Igarashi, T. Okajima, R. Utsumi, E. Kinoshita and T. Koike.
Electrophoresis. 2019 Nov;40(22):3005-3013.

Total synthesis of Caprazamycin A: Practical and scalable synthesis of syn-β-Hydroxyamino acids and introduction of a fatty acid side chain to 1,4-Diazepanone.
H. Nakamura , C. Tsukano, T. Yoshida, M. Yasui, S. Yokouchi, Y. Kobayashi, M. Igarashi and Y. Takemoto.
J. Am. Chem. Soc. 2019 May 29;141(21):8527-8540.

Total Synthesis of Pactalactam, an Imidazolidinone-Type Pactamycin Analogue.
T. Kim, S. Matsushita, S. Matsudaira, T. Doi, S. Hirota,Y. T. Park, M. Igarashi, M. Hatano, N. Ikeda, J. Ham, M. Nakata and Y. Saikawa.
Org Lett. 2019 May 17;21(10):3554-3557.

New natural products to meet the antibiotic crisis: a personal journey.
M. Igarashi.
J Antibiot. 2019 Dec;72(12):890-898.

Systematic and stereoselective total synthesis of mannosylerythritol lipids and evaluation of their antibacterial activity.
J. Nashida, N. Nishi, Y. Takahashi, C. Hayashi, M. Igarashi, D. Takahashi and K. Toshima
J. Org. Chem. 2018, 83, 7281-7289

Instability of the 16S rRNA methyltransferase-encoding npmA gene: why have bacterial cells possessing npmA not spread despite their high and broad resistance to aminoglycosides?
Y. Ishizaki, Y. Shibuya, C. Hayashi, K. Inoue, T. Kirikae, T. Tada, T. Miyoshi-Akiyama and M. Igarashi
J. Antibiot. 2018, 71, 798-807

Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection.
T. Ohishi, T. Masuda, H. Abe, C. Hayashi, H. Adachi, S. Ohba, M. Igarashi, T. Watanabe, H. Mimuro, E. Amalia, DK. Inaoka, K. Mochizuki, K. Kita, M. Shibasaki and M. Kawada
Helicobacter 2018, 23, e12470

Quadoctomycin, a 48-membered macrolide antibiotic from Streptomyces sp.MM168-141F8.
R. Sawa, Y. Kubota, M. Umekita, M. Hatano, C. Hayashi and M. Igarashi.
J. Antibiot. 2018, 71, 91-96

A guanine derivative as a new MEK inhibitor produced by Streptomyces sp. MK63-43F2.
M. Iijima, Yuji Kubota, R. Sawa, Yumiko Kubota, M. Hatano, M. Igarashi, M. Kawada, I. Momose, M. Takekawa and M. Shibasaki
J. Antibiot. 2018, 71, 135-138

Valgamicin C, a novel cyclic depsipeptide containing the unusual amino acid cleonine, and related valgamicins A, T and V produced by Amycolatopsis sp. ML1-hF4.
H. Hashizume, K. Iijima, K. Yamashita, T. Kimura, S. Wada, R. Sawa and M. Igarashi
J. Antibiot. 2018, 71, 129-134

ATP Depletion Assay Led to the Isolation of New 36-Membered Polyol Macrolides Deplelides A and B from Streptomyces sp. MM581-NF15.
T. Takeuchi, M. Hatano, M. Umekita, C. Hayashi, S. Wada, M. Nagayoshi, R. Sawa, Y. Kubota, M. Kawada, M. Igarashi and M. Shibasaki
Org. Lett. 2017, 19, 4207-4210

In vivo efficacy of β-lactam/tripropeptin C in a mouse septicemia model and the mechanism of reverse β-lactam resistance in methicillin-resistant Staphylococcus aureus mediated by tripropeptin C.
H. Hashizume, Y. Takahashi, T. Masuda, S. Ohba, T. Ohishi, M. Kawada and M. Igarashi
J. Antibiot. 2018, 71, 79-85

Fradiamine A, a new siderophore from the deep-sea actinomycete Streptomyces fradiae MM456M-mF7.
Y. Takehana, M. Umekita, M. Hatano, C. Kato, R. Sawa, and M. Igarashi
J. Antibiot. 2017, 70, 611-615

Structures and biological activities of novel 4’-acetylated analogs of chrysomycins A and B.
S. Wada, R. Sawa, F. Iwanami, M. Nagayoshi, Y. Kubota, K. Iijima, C. Hayashi, Y. Shibuya, M. Hatano, M. Igarashi and M. Kawada
J. Antibiot. 2017, 70, 1078-1082

薬剤耐性と創薬研究
－アミノグリコシド系抗生物質を中心として－
五十嵐　雅之、高橋　良昭
Obe-lisk 2017, 22, 7-12.

Structure and antibacterial activities of new cyclic peptide antibiotics, pargamicins B, C and D, from Amycolatopsis sp. ML1-hF4
H. Hashizume, R. Sawa, K. Yamashita, Y. Nishimura and M. Igarashi.
J. Antibiot. 2017, 70, 699-704

Epithelial-mesenchymal transition promotes SOX2 and NANOG expression in bladder cancer.
T. Migita, A. Ueda, T. Ohishi, M. Hatano, H. Seimiya, S. Horiguchi, F. Koga and F. Shibasaki.
Laboratory Investigation 2017, 97, 567-576

Stability and bioavailability of lentztrehaloses A, B, and C as replacements for trehalose.
S. Wada, R. Sawa, S. Ohba, C. Hayashi, M. Umekita, Y. Shibuya, K. Iijima, F. Iwanami and M. Igarashi.
J. Agric. Food Chem. 2016, 64, 7121-7126.

Synthesis and antibacterial activity of tripropeptin C derivatives containing the pyrimidine ring system.
S. Hirosawa, Y. Takahashi, A. Murotani, H. Hashizume, T. Miyake and Y. Akamatsu.
Heterocycles 2015, 91, 2143-2151.

2014年　浜田賞受賞論文　放線菌を初めとする土壌細菌由来の有用抗菌物質の探索と作用機序解析
橋爪秀樹
日本放線菌学会誌 2015, 29, 8-12

Novel autophagy inducers lentztrehaloses A, B and C
S. Wada, Y. Kubota, R. Sawa, M. Umekita, M. Hatano, S. Ohba, C. Hayashi, M. Igarashi and A. Nomoto.
J. Antibiot. 2015, 68, 521-529

Natural lipopeptide antibiotic tripropeptin C revitalizes and synergistically potentiates the activity
of beta-lactams against methicillin-resistant Staphylococcus aureus.
H. Hashizume, Y. Takahashi, S. Harada and A. Nomoto.
J. Antibiot. 2015, 68, 373-378

Androprostamines A and B, the new anti-prostate cancer agents produced by Streptomyces sp. MK932-CF8.
Y. Yamazaki, T. Someno, M. Igarashi, N. Kinoshita, M. Hatano, M. Kawada, I.Momose and
A. Nomoto.
J. Antibiot. 2014, 68, 279-285

Synthesis and antibacterial activity of tripropeptin C derivatives modified at the carboxyl groups.
S. Hirosawa, Y. Takahashi, H. Hashizume, T. Miyake and Y. Akamatsu.
J. Antibiot. 2014, 67, 265-268.

Structure and biological properties of lentztrehalose: a novel trehalose analog.
S. Wada, S. Ohba, T. Someno, M. Hatano and A. Nomoto.
J. Antibiot. 2013, 67, 319-322.

Waldiomycin, a novel WalK-histidine kinase inhibitor from Streptomyces sp. MK844-mF10.
M. Igarashi, T. Watanabe, T. Hashida, M. Umekita, M. Hatano, Y. Yanagida, H. Kino, T. Kimura, N. Kinoshita, K. Inoue, R. Sawa, Y. Nishimura, R. Utsumi and A. Nomoto.
J. Antibiot. 2013, 66, 459-464.

Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood coagulation by binding to prothrombin and factor Xa via their γ-carboxyglutamic acid (Gla) domain.
S. Itoh, R. Yokoyama, G. Kamoshida, T. Fujiwara, H. Okada, T. Takii, T. Tsuji, S. Fujii,
H. Hashizume and K. Onozaki.
J. Biol. Chem. 2013, 288, 21569-21580.

Inhibition of the first step in synthesis of the mycobacterial cell wall core, catalyzed by the GlcNAc-1-phosphate transferase WecA, by the novel caprazamycin derivative CPZEN-45.
Y. Ishizaki, C. Hayashi, K. Inoue, M. Igarashi, Y. Takahashi, V. Pujari, D. C. Crick, P. J. Brennan and A. Nomoto.
J. Biol. Chem. 2013, 288, 30309-30319.

Structure-activity relationship study of intervenolin derivatives:
synthesis, antitumor and anti-Helicobacter Pylori activities.
H. Abe, M. Kawada, H. Inoue, S. Ohba, T. Masuda, C. Hayashi, M. Igarashi,
A. Nomoto, T. Watanabe and M. Shibasaki.
Tetrahedron 2013, 69, 7608-7617.

Intervenolin, a new antitumor compound with anti-Helicobacter pylori activity, from Nocardia sp. ML96-86F2.
M. Kawada, H. Inoue, S. Ohba, M. Hatano, M. Amemiya, C. Hayashi, I. Usami, H. Abe, T. Watanabe, N. Kinoshita, M. Igarashi, T. Masuda, D. Ikeda and A. Nomoto.
J. Antibiot. 2013, 66, 543-548.

Sacchathridine A, a prostaglandin release inhibitor from Saccharothrix sp.
K. Nakae, I. Kurata, F. Kojima, M. Igarashi, M. Hatano, R. Sawa, Y. Kubota,
H. Adachi and A. Nomoto.
J. Natural Products 2013, 76, 720-722.

Vegfrecine, an inhibitor of VEGF receptor tyrosine kinases isolated
from the culture broth of Streptomyces sp.
C. Nosaka, H. Adachi, R. Sawa, K. Nakae, S. Atsumi, N. Kinoshita, Y. Kubota,
M. Igarashi, Y. Sei, K. Yamaguchi, M. Shibuya, Y. Nishimura and Y. Akamatsu.
J. Natural Products 2013, 76, 715-719.

NAD(P)H quinone oxidoreductase 1 (NQO1)-bioactivated pronqodine A
regulates prostaglandin release from human synovial sarcoma cells.
K. Nakae, H. Adachi, R. Sawa, N. Hosokawa, M. Hatano, M. Igarashi,
Y. Nishimura, Y. Akamatsu and A. Nomoto.
J. Natural Products 2013, 76, 510-515.

Novel semisynthetic antibiotics from caprazamycins A-G: caprazene derivatives
and their antibacterial activity.
Y. Takahashi, M. Igarashi, T. Miyake, H. Soutome, K. Ishikawa, Y. Komatsuki,
Y. Koyama, N. Nakagawa, S. Hattori,K. Inoue, N. Doi and Y. Akamatsu.
J. Antibiot. 2013, 66, 171-178.

Amycolamicin: a novel broad-spectrum antibiotic inhibiting bacterial topoisomerase.
R. Sawa, Y. Takahashi, H. Hashizume,　K. Sasaki,　Y. Ishizaki,　M.Umekita,　M. Hatano,
H. Abe,　T. Watanabe,　N. Kinoshita,　Y. Homma, C. Hayashi,　K. Inoue,　S. Ohba,
T. Masuda,　 M. Arakawa,　Y. Kobayashi,　M. Hamada,　M. Igarashi,　H. Adachi,
Y. Nishimura and Y. Akamatsu.
Chem. Eur. J. 2012, 18, 15772-15781.

Discovery and characterization of NK13650s, naturally occurring
p300-selective histone acetyltransferase inhibitors.
S. Tohyama, A. Tomura, N. Ikeda, M. Hatano, J. Odanaka, Y. Kubota,
M.Umekita, M. Igarashi, R. Sawa and T. Morino.
J. Org. Chem. 2012, 77, 9044-9052.

Curing bacteria of antibiotic resistance: reverse antibiotics, a novel
class of antibiotics in nature.
K. Hiramatsu, M. Igarashi, Y. Morimoto, T. Baba, M. Umekita and Y. Akamatsu.
Int. J. Antimicrob. Agents. 2012, 39, 478-485.

Application of matrix-assisted laser desorption ionization-time of
flight mass spectrometry for discrimination of laboratory-derived
antibiotic-resistant bacteria.
M. Muroi, K. Shima, M. Igarashi, Y. Nakagawa and K. Tanamoto.
Biol. Pharm. Bull. 2012, 35, 1841-1845.

Isolation and characterization of signamycine B, an antibiotic that targets the dimerization domain of histidine kinase WalK.
T. Watanabe, M. Igarashi, T. Okajima, E. Ishii, H. Kino, M. Hatano, R.Sawa,
M. Umekita, T. Kimura, S. Okamoto,Y. Eguchi, Y. Akamatsu and R. Utsumi.
Antimicrob. Agents Chemother. 2012, 56, 3657-3663.

Decalpenic acid induces early osteoblastic markers in pluripotent
mesenchymal cells via activation of retinoic acid receptor gamma.
S. Sakamoto, F. Kojima, I. Momose, M. Kawada, H. Adachi and Y. Nishimura.
Biochem. Biophys. Res. Commun. 2012, 422, 751-757.

抗メチシリン耐性黄色ブドウ球菌薬の開発研究
橋爪秀樹
薬学雑誌 2012, 132, 59-67.

病原性細菌に対する新しい薬剤標的－二成分情報伝達システム
内海龍太郎、五十嵐雅之
薬学雑誌 2012, 132, 51-58.

新しい抗結核薬の開発状況
五十嵐雅之、高橋良昭
日本臨牀：結核とその類縁疾患 2011, 69, 1482-1488.

Tripropeptin C blocks the lipid cycle of cell wall biosynthesis by complex formation with
undecaprenyl pyrophosphate.
H. Hashizume, R. Sawa, S. Harada, M. Igarashi, H. Adachi, Y. Nishimura and
A. Nomoto.
Antimicrob. Agents Chemother. 2011, 55, 3821-3828.

Development of an antivirulence drug against Streptococcus mutans: repression of biofilm formation, acid tolerance, and competence by a histidine kinase inhibitor, Walkmycin C.
Y. Eguchi, N. Kubo, H. Matsunaga, M. Igarashi and R. Utsumi.
Antimicrob. Agents Chemother. 2011, 55, 1475-1484.

Photodegradation of target oligosaccharides by light-activated small molecules.
D. Takahashi, S. Hirono, C. Hayashi, M. Igarashi, Y Nishimura and K. Toshima.
Angew. Chem. Int. Ed. 2010, 49, 10096-10100.

Decalpenic acid, a novel small molecule from Penicillium verruculosum CR37010,
induces early osteoblastic markers in pluripotent mesenchymal cells.
S. Sakamoto, F. Kojima, M. Igarashi, R. Sawa, M. Umekita, Y. Kubota, K. Nakae,
S. Yamaguchi, H. Adachi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 703-708.

Isolation and structure elucidation of a novel androgen antagonist, arabilin, produced
by Streptomyces sp. MK756-CF1.
T. Kawamura, T. Fujimaki, N. Hamanaka, K. Torii, H. Kobayashi, Y. Takahashi,
M. Igarashi, N. Kinoshita, Y. Nishimura, E. Tashiro and M. Imoto.
J. Antibiot. 2010, 63, 601-605.

The synthesis of paleic acid, an antimicrobial agent effective against Mannheimia and
Pasteurella, and its structurally related derivatives.
T. Watanabe, I. Kurata, C. Hayashi, M. Igarashi, R. Sawa, Y. Takahashi and
Y. Akamatsu.
Bioorg. Med. Chem. Lett. 2010, 20, 5843-5846.

Biological activities of pargamicin A, a novel cyclic peptide antibiotic from
Amycolatopsis sp.
H. Hashizume, H. Adachi, M. Igarashi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 279-283.

Biosynthesis of amycolamicin: the biosynthetic origin of a branched α-aminoethyl
moiety in the unusual sugar amycolose.
S. Tohyama, Y. Takahashi and Y. Akamatsu.
J. Antibiot. 2010, 63, 147-149.

Novel antibacterial compounds specifically targeting the essential WalR response
regulator.
Y. Gotoh, A. Doi, E. Furuta, S. Dubrac, Y. Ishizaki, M. Okada, M. Igarashi, N. Misawa,
H. Yoshikawa, T. Okajima, T. Msadek and R. Utsumi.
J. Antibiot. 2010, 63, 127-134.

Antipain Y, a new antipain analog that inhibits neurotransmitter release from rat
dorsal root ganglion neurons.
K. Nakae, F. Kojima, R. Sawa, Y. Kubota, M. Igarashi, N. Kinoshita, H. Adachi,
Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 41-44.

Identification of the toxic trigger in mushroom poisoning.
M. Matsuura, Y. Saikawa, K. Inui, K. Nakae, M. Igarashi, K. Hashimoto and
M. Nakata.
Nat. Chem. Biol. 2009, 5, 465-467.

High-throughput screening assay of hepatitis C virus helicase inhibitors using
fluorescence-quenching phenomenon.
H. Tani, N. Akimitsu, O. Fujita, Y. Matsuda, R. Miyata, S. Tsuneda, M. Igarashi,
Y. Sekiguchi and N. Noda.
Biochem. Biophys. Res. Commun. 2009, 379, 1054-1059.

Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner
independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells.
K. Kadohara, M. Nagumo, S. Asami, Y. Tsukumo, H. Sugimoto, M. Igarashi,
K. Nagai and T. Kataoka.
J. Biol. Chem. 2009, 284, 5478-5487.

A new type of tripropeptin with anteiso-branched chain fatty acid from Lysobacter sp.
BMK333-48F3.
H. Hashizume, M. Igarashi, R. Sawa, H. Adachi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2008, 61, 577-582.

ERK and p38 MAP kinase are involved in downregulation of cell surface TNF receptor 1
induced by acetoxycycloheximide.
H. Ogura, Y. Tsukumo, H. Sugimoto, M. Igarashi, K. Nagai and T. Kataoka.
Int. Immunopharmacol. 2008, 8, 922-926.

Ectodomain shedding of TNF receptor 1 induced by protein synthesis inhibitors regulates
TNF-α-mediated activation of NF-κB and caspase-8.
H. Ogura, Y. Tsukumo, H. Sugimoto, M. Igarashi, K. Nagai and T. Kataoka.
Exp. Cell Res. 2008, 314, 1406-1414.

Discovery of incednine as a potent modulator of the anti-apoptotic function of Bcl-xL from microbial origin.
Y. Futamura, R. Sawa, Y. Umezawa, M. Igarashi, H. Nakamura, K. Hasegawa,
M. Yamasaki, E. Tashiro, Y. Takahashi, Y. Akamatsu and M. Imoto.
J. Am. Chem. Soc. 2008, 130, 1822-1823.