Section of Animal Resources

Research Outline

In vivo evaluation of the compounds isolated from microbial metabolites or synthesized in the laboratory.

Animal Evaluation System Outline

1. Toxicity and PK/PD studies

①Acute toxicity
  • Weight changes
  • External changes
  • Anatomy findings
    • Observation for 14 days
②Pharmacokinetic examination
  • Serum
  • Urine
  • Excrement

2. Antitumor studies

①Antitumor examination
Human transplantable tumor
  • Lung : LX-1, DMS-273
  • Pancreatic : Capan-1, PANC-1
  • Colon : DLD-1
  • Prostate : LNCaP, PC-3, DU-145
  • Breast : BSY-1
  • Melanoma : LOX
  • Others
Mouse transplantable tumor
  • Ehrlich carcinoma
  • IMC carcinoma
  • Sarcoma : S180
  • Fibro sarcoma : Meth-A
  • Leukemia : L1210, EL4
  • Colon cancer : Colon26
  • Melanoma : B16-F10, B16-BL6
  • Others
②Antiangiogenic examination
Fig.1 Model of the tumor angiogenesis.
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③Antimetastatic examination
Fig.2 Experimental and spontaneous metastasis model.
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3. Antibacteria study

Model of the systemic infection
Staphylococcus aureus Smith
MRSA
Staphylococcus aureus MS5935-3
Staphylococcus aureus BASA56

Fig.3 Model of the systemic infection caused by Staphylococcus aureus Smith.
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4. Antiarthritic study

To induce the arthritis, type II collagen complete adjuvant (FCA) is inoculated to the mouse on day 0 and 21. Footpad volumes and joint changes of limb are measured.

The experiment is performed in accordance with the Guideline for Animal Experiment in the MCRF.