Section of Animal Resources

Research Outline

In vivo evaluation of the compounds isolated from microbial metabolites or synthesized in the laboratory.

Animal Evaluation System Outline

1. Toxicity and PK/PD studies

①Acute toxicity
  • Weight changes
  • External changes
  • Anatomy findings
    • Observation for 14 days
②Pharmacokinetic examination
  • Serum
  • Urine
  • Excrement

2. Antitumor studies

①Antitumor examination
Human transplantable tumor
  • Lung : LX-1, DMS-273
  • Pancreatic : Capan-1, PANC-1
  • Colon : DLD-1
  • Prostate : LNCaP, PC-3, DU-145
  • Breast : BSY-1
  • Melanoma : LOX
  • Others
Mouse transplantable tumor
  • Ehrlich carcinoma
  • IMC carcinoma
  • Sarcoma : S180
  • Fibro sarcoma : Meth-A
  • Leukemia : L1210, EL4
  • Colon cancer : Colon26
  • Melanoma : B16-F10, B16-BL6
  • Others
②Antiangiogenic examination
Fig.1 Model of the tumor angiogenesis.
③Antimetastatic examination
Fig.2 Experimental and spontaneous metastasis model.

3. Antibacteria study

Model of the systemic infection
Staphylococcus aureus Smith
Staphylococcus aureus MS5935-3
Staphylococcus aureus BASA56

Fig.3 Model of the systemic infection caused by Staphylococcus aureus Smith.

4. Antiarthritic study

To induce the arthritis, type II collagen complete adjuvant (FCA) is inoculated to the mouse on day 0 and 21. Footpad volumes and joint changes of limb are measured.

The experiment is performed in accordance with the Guideline for Animal Experiment in the MCRF.